| Solution Information | help | |
| Enzyme: | D(1B) dopamine receptor | |
| inhibitor: | BDBM48936 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | The goal of this screening campaign was to test confirmed active compounds for their ability to modulate the D5 receptor in a dose-dependent fashion in the presence of an EC30 of dopamine. Samples were tested in triplicate for this assay. Assay Description: Signaling through the D5 receptor is coupled to the CRE/luciferase reporter system in HEK 293 cells. Compounds at 10 micromolar final concentration were tested in duplicate for their ability to modulate the D5 receptor in the presence of dopamine stimulation. The signal readout from this assay is luciferase luminescence as measured by the Perkin Elmer Envision. Assay Protocol: 1. Human Embryonic Kidney Cells (HEKs) containing human D5 receptor were seeded at 35000 cells/well in Greiner black, solid bottom poly-lysine coated 384-well plates (Cat#781947) in Dulbecco's modified medium (DMEM) containing 5% Fetalclone I serum (Hyclone), 5% iron-supplemented bovine calf serum (Hyclone), 0.05 U/ml penicillin, and 50 mg/ml streptomy | |
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